Journal: Biology of Sex Differences

Article Title: Sex differences in disease severity and immune responses in murine and human inflammatory arthritis

doi: 10.1186/s13293-026-00840-w

Figure Lengend Snippet: Male CIA mice exhibit higher disease severity compared to females. Male and female CIA and saline control mice were monitored for disease severity and assigned clinical scores from day 21 after the first CII challenge until the end of experiment (day 29). (A) Line graphs representing the mean clinical scores of mice starting from day 1 to day 29. On day 29 after the first CII challenge, mice were euthanized by cardiac puncture under anesthesia for blood and serum collection and storage at −80 °C. Serum concentrations of (B) anti-mouse collagen type II antibodies (left panel) and anti-bovine collagen type II antibodies (right panel) were analyzed by ELISA. N = 5 mice per group. One of two independent experiments. Simple linear regression analysis was performed to determine the statistical difference between the lines. One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test was used to determine the statistical significance between the groups. (*/ # p ≤ 0.05, ** p ≤ 0.005 and ***/ ### p ≤ 0.0005). In Fig. 1A, # significance of comparisons between CIA and saline control mice; *significance of comparisons between sexes of CIA mice

Article Snippet: Serum levels of mouse anti-collagen antibodies (autoantibodies) and bovine anti-collagen antibodies (antibodies to the immunizing antigen) were determined by ELISA using a Mouse Anti-mouse Type II Collagen IgG Antibody Assay Kit and Mouse Anti-Bovine Type II Collagen IgG Antibody Assay Kit, respectively, according to the manufacture’s protocol (Chondrex Inc. WA, USA).

Techniques: Saline, Control, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Annals of biomedical engineering

Article Title: Hydrostatic pressure differentially regulates outer and inner annulus fibrosus cell matrix production in 3D scaffolds.

doi: 10.1007/s10439-007-9407-6

Figure Lengend Snippet: FIGURE 5. Normalized COL II content of outer annulus (a) and inner annulus (b) cell-seeded constructs (n = 4). Significance set at p < 0.05. * Significant effect of loading condition within time point. # Significant effect of time within loading condition. ^ Significant vs. bagged control within time point.

Article Snippet: Protein values for each sample were determined using a standard curve generated from bovine COL I and COL II (Rockland Immunochemicals, Gilbertsville, PA).

Techniques: Construct, Control

Journal: Annals of biomedical engineering

Article Title: Hydrostatic pressure differentially regulates outer and inner annulus fibrosus cell matrix production in 3D scaffolds.

doi: 10.1007/s10439-007-9407-6

Figure Lengend Snippet: FIGURE 7. COL II immunohistochemical staining of outer annulus (a–c) and inner annulus (d–f) cell-seeded constructs at 14 days. Culture conditions indicated by column heading. Arrows indicate presence of collagen fibril networks, which are more pronounced in panel c. Bar = 50 lm.

Article Snippet: Protein values for each sample were determined using a standard curve generated from bovine COL I and COL II (Rockland Immunochemicals, Gilbertsville, PA).

Techniques: Immunohistochemical staining, Staining, Construct